P17. RIG-I-like helicases induce immunogenic cell death of pancreatic cancer cells
نویسندگان
چکیده
Results RLH ligands induced production of type I IFN, HMGB1 and Hsp70 and translocation of calreticulin to the outer cell membrane of tumour cells. In cocultures, DC upregulated B7 expression, which was mediated by tumourderived type I IFN, whereas TLR, RAGE or inflammasome signaling was dispensable. CD8a DC effectively engulfed apoptotic tumour material and cross-presented tumourassociated antigen to naïve CD8 T cells. In comparison, tumour cell death mediated by oxaliplatin, staurosporine or mechanical disruption failed to induce DC activation, antigen uptake or cross-presentation. Moreover, tumour cells treated with sublethal doses of RLH ligands upregulated MHC-I and Fas expression and were sensitised towards CTLand Fas-mediated killing.
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