Characterization of lignin-degrading enzymes (LDEs) from a dimorphic novel fungus and identification of products of enzymatic breakdown of lignin.

Determination of lignin-modifying enzymes (LMEs) in Hyphodermella species using biochemical and molecular techniques

White-rot basidiomycetes are one of the most important lignolytic microorganisms. These fungi have been reported to secrete three main classes of lignin degrading enzymes: lignin peroxidases (LiPs), manganese peroxidases (MnPs) and laccases. In this study, for the first time the lignin degrading capability of two plant pathogens i.e. Hyphodermella rosae and H. corrugata was evaluated using both...

Genome sequence and description of the anaerobic lignin-degrading bacterium Tolumonas lignolytica sp. nov.

Tolumonas lignolytica BRL6-1(T) sp. nov. is the type strain of T. lignolytica sp. nov., a proposed novel species of the Tolumonas genus. This strain was isolated from tropical rainforest soils based on its ability to utilize lignin as a sole carbon source. Cells of Tolumonas lignolytica BRL6-1(T) are mesophilic, non-spore forming, Gram-negative rods that are oxidase and catalase negative. The g...

Development of Novel Assays for Lignin Breakdown and Identification of a New Bacterial Lignin Degrading Enzyme

ISEN METAGENOMIC DISCOVERY OF NOVEL LIGNIN - DEGRADING FUNGI FOR BIOFUEL PRODUCTION Louise

BACKGROUND AND OBJECTIVES The bio-conversion of plant lignocellulose to glucose is an key component of second generation biofuel production, but the resistance of lignin to breakdown is a major obstacle in this process. Filamentous fungi possess the unique ability to decompose the aromatic lignin polymers using enzymes encoded by divergent gene families. White-rot fungi are the primary lignin-d...

Lignin-Degrading Enzymes of the Commercial Button Mushroom, Agaricus bisporus.

Agaricus bisporus, grown under standard composting conditions, was evaluated for its ability to produce lignin-degrading peroxidases, which have been shown to have an integral role in lignin degradation by wood-rotting fungi. The activity of manganese peroxidase was monitored throughout the production cycle of the fungus, from the time of colonization of the compost through the development of f...