Inhhibitory effects of saturated fatty acids on methane production by methanogenic Archaea

The present study investigated the inhibitory effects of saturated fatty acids on methanogenesis in Archaea, and whether or not competitive inactivation of the methanogens’ coenzyme M (HS-CoM) is involved in the inhibition. Strains tested in batch cultures were Methanosarcina barkeri, Methanosarcina mazei, Methanococcus voltae, all incubated at 37°C, and Methanothermobacter thermoautotrophicus, incubated at 65°C. The fatty acids C10, C12, C14 and C18 were supplemented at 1 mg . ml–1 in cultivation medium. The methanogens were susceptible to C10 and C12, and less so to C14. Only M. thermoautotrophicus was affected by C18. In M. mazei cultures, excessive HS-CoM did not prevent the action of C14 which might suggest that competitive inhibition of HS-CoM is not the reason for the SFAinduced effect on methanogenesis. The results indicate that, as a prerequisite to inhibit methanogenesis in Archaea, mediumand long-chain saturated fatty acids have to be at least partially molten. Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-78626 Published Version Originally published at: Zeitz, J O; Bucher, S; Zhou, X; Meile, L; Kreuzer, M; Soliva, C R (2013). Inhhibitory effects of saturated fatty acids on methane production by methanogenic Archaea. Journal of Animal and Feed Sciences, 22(1):44-49. Journal of Animal and Feed Sciences, Vol. 22, No. 1, 2013, 44–49 The Kielanowski Institute of Animal Physiology and Nutrition PAS Jabłonna Inhibitory effects of saturated fatty acids on methane production by methanogenic Archaea J.O. Zeitz1,4, S. Bucher1, X. Zhou1, L. Meile2, M. Kreuzer1 and C.R. Soliva1,3 1 ETH Zurich, Institute of Agricultural Sciences, Universitaetstrasse 2, 8092 Zurich, Switzerland 2 ETH Zurich, Institute of Food, Nutrition and Health, Schmelzbergstr. 7, 8092 Zurich, Switzerland 3 Current address: University of Zurich, Institute of Animal Nutrition, Winterthurerstrasse 260, 8057 Zurich, Switzerland