Microfluidics for Control in Synthetic Biology
نویسندگان
چکیده
We report the microfluidic control of a synthetic biological system using a one milliliter volume microbioreactor. Precise fluid injection and mixing enable control schemes such as rapid medium introduction in continuous culture conditions. A Saccharomyces cerevisiae strain with a dual inducible fluorescent reporter system was tested with the microbioreactor cultivation for precise induction control. Flow cytometry data suggests that both higher gene expression levels and tighter population distributions were readily achievable with the microbioreactor compared to test tube induction.
منابع مشابه
Opportunities for microfluidic technologies in synthetic biology.
We introduce microfluidics technologies as a key foundational technology for synthetic biology experimentation. Recent advances in the field of microfluidics are reviewed and the potential of such a technological platform to support the rapid development of synthetic biology solutions is discussed.
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