The assembly domain of the small capsid protein of Kaposi's sarcoma-associated herpesvirus.

نویسندگان

  • Dale Kreitler
  • Christopher M Capuano
  • Brandon W Henson
  • Erin N Pryce
  • Daniel Anacker
  • J Michael McCaffery
  • Prashant J Desai
چکیده

Self-assembly of Kaposi's sarcoma-associated herpesvirus capsids occurs when six proteins are coexpressed in insect cells using recombinant baculoviruses; however, if the small capsid protein (SCP) is omitted from the coinfection, assembly does not occur. Herein we delineate and identify precisely the assembly domain and the residues of SCP required for assembly. Hence, six residues, R14, D18, V25, R46, G66, and R70 in the assembly domain, when changed to alanine, completely abolish or reduce capsid assembly.

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عنوان ژورنال:
  • Journal of virology

دوره 86 21  شماره 

صفحات  -

تاریخ انتشار 2012