Flow-through, Viral Co-infection Assay for Low Resource Settings
نویسنده
چکیده
Here we present a new, rapid, immunofiltration assay for simultaneous detection of HIV p24 and Hepatitis B Virus antigens. The assay platform is composed of a 13 mm nitrocellulose filter spotted with capturing bioprobes and inserted in a Swinnex syringe filter holder. Samples and reagents are flown through the nitrocellulose filter by manual pressure on the syringe. A colorimetric detection allows for naked eye results interpretation. The assay provides sensitivity in the picomolar range in just 5 minutes, even using low volumes of sample in complex matrix.
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