Dissemination of the tetM tetracycline resistance determinant to Ureaplasma urealyticum.
نویسندگان
چکیده
Ureaplasma urealyticum is an organism considered susceptible to tetracycline. Ten tetracycline-resistant (Tcr) clinical isolates and Tcr serotype 9 were examined. All contained DNA sequences homologous to the streptococcal determinant tetM. They differed from each other on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Southern blots, and immunoblots and appeared to represent different strains.
منابع مشابه
Cloning and characterization of tetM gene from a Ureaplasma urealyticum strain.
We have cloned a 4.9-kilobase (kb) HincII fragment which contains a tetracycline resistance determinant (tetM) from the chromosome of Ureaplasma urealyticum. The 4.9-kb HincII fragment contains DNA in addition to the structural gene, is closely related to the previously characterized 5.0-kb fragment from pJI3, and contains no normal ureaplasmal DNA sequences.
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The nucleotide sequence of the tetracycline resistance gene tetM encoded by streptococcal conjugative shuttle transposon Tnl545 has been determined. The resistance gene was identified as a coding sequence of 1917 base pairs corresponding to a protein with a Mj. of 72,500 daltons. This value is in good agreement with that, 68,000 daltons, estimated by SDS-polyacrylamide gel electrophoresls of Es...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 29 2 شماره
صفحات -
تاریخ انتشار 1986