Effects of preanalytical factors on the molecular size of cell-free DNA in blood.

نویسندگان

  • K C Allen Chan
  • Sze-Wan Yeung
  • Wing-Bong Lui
  • Timothy H Rainer
  • Y M Dennis Lo
چکیده

also see Table 2 in the online Data Supplement). In HRT users, we found no significant differences in serum cobalamin or in plasma holoTC, MMA, and tHcy compared with controls. To our knowledge, there are currently no published data on the effects of HRT on plasma holoTC, and studies on serum cobalamin have not established a clear pattern (4, 5). We observed that HRT use decreased the risk of having plasma MMA in the highest quartile but was not associated with significant changes in plasma MMA (Table 1). In another study investigating postmenopausal women Ͼ60 years, HRT users had significantly lower plasma MMA than nonusers (5). Oral HRT lowered (20) or did not affect plasma tHcy (4). Our findings indicate at least that use of HRT has no negative effect on cobalamin status. In conclusion, both serum cobalamin and holoTC are lower by 25% in younger women taking OCs, but this is not associated with significantly higher concentrations of the metabolic markers of impaired cobalamin status, plasma MMA and plasma tHcy. This may suggest redistribution rather than depletion of intracellular cobalamin. Such hormonal effects may weaken the diagnostic utility of total cobalamin and holoTC. Further studies are warranted to decide whether OC users with marginal cobal-amin status are prone to develop cobalamin deficiency. HRT use had no noticeable effect on circulating cobal-amin, holoTC, or the metabolic markers. homocysteine in women on oral estrogen-containing contraceptives and in men with estrogen-treated prostatic carcinoma. Effects of oral and transdermal 17␤-estradiol combined with progesterone on homocysteine metabolism in postmenopausal women: a randomised placebo-controlled trial. Simultaneous profile analysis of plasma amino and organic acids by capillary gas chromatography. Effects of oral contraceptives and hormone replacement therapy on cobalamin status [Abstract]. Stilgren L, et al. Effect of long-term hormone replacement therapy on plasma homocysteine in postmenopausal women: A randomized controlled study. Recently, plasma DNA analysis has been increasingly explored for different clinical diagnostic purposes. Previous studies have shown that the concentration of plasma Fig. 1. Dose–response relationships of plasma holoTC and serum cobalamin with the metabolic markers plasma MMA and plasma tHcy, according to OC use. The curves were obtained by additive gaussian generalized models. The models include age, serum creatinine, and smoking. The solid lines are the estimated dose–response curves, and the shaded areas are the 95% CIs. The P values indicate the significance of the smooth terms.

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عنوان ژورنال:
  • Clinical chemistry

دوره 51 4  شماره 

صفحات  -

تاریخ انتشار 2005