Effects of preanalytical factors on the molecular size of cell-free DNA in blood.
نویسندگان
چکیده
also see Table 2 in the online Data Supplement). In HRT users, we found no significant differences in serum cobalamin or in plasma holoTC, MMA, and tHcy compared with controls. To our knowledge, there are currently no published data on the effects of HRT on plasma holoTC, and studies on serum cobalamin have not established a clear pattern (4, 5). We observed that HRT use decreased the risk of having plasma MMA in the highest quartile but was not associated with significant changes in plasma MMA (Table 1). In another study investigating postmenopausal women Ͼ60 years, HRT users had significantly lower plasma MMA than nonusers (5). Oral HRT lowered (20) or did not affect plasma tHcy (4). Our findings indicate at least that use of HRT has no negative effect on cobalamin status. In conclusion, both serum cobalamin and holoTC are lower by 25% in younger women taking OCs, but this is not associated with significantly higher concentrations of the metabolic markers of impaired cobalamin status, plasma MMA and plasma tHcy. This may suggest redistribution rather than depletion of intracellular cobalamin. Such hormonal effects may weaken the diagnostic utility of total cobalamin and holoTC. Further studies are warranted to decide whether OC users with marginal cobal-amin status are prone to develop cobalamin deficiency. HRT use had no noticeable effect on circulating cobal-amin, holoTC, or the metabolic markers. homocysteine in women on oral estrogen-containing contraceptives and in men with estrogen-treated prostatic carcinoma. Effects of oral and transdermal 17-estradiol combined with progesterone on homocysteine metabolism in postmenopausal women: a randomised placebo-controlled trial. Simultaneous profile analysis of plasma amino and organic acids by capillary gas chromatography. Effects of oral contraceptives and hormone replacement therapy on cobalamin status [Abstract]. Stilgren L, et al. Effect of long-term hormone replacement therapy on plasma homocysteine in postmenopausal women: A randomized controlled study. Recently, plasma DNA analysis has been increasingly explored for different clinical diagnostic purposes. Previous studies have shown that the concentration of plasma Fig. 1. Dose–response relationships of plasma holoTC and serum cobalamin with the metabolic markers plasma MMA and plasma tHcy, according to OC use. The curves were obtained by additive gaussian generalized models. The models include age, serum creatinine, and smoking. The solid lines are the estimated dose–response curves, and the shaded areas are the 95% CIs. The P values indicate the significance of the smooth terms.
منابع مشابه
Effects of Preanalytical Factors on the Molecular Size of Cell-Free DNA in Blood, K.C
Cell-Free DNA in Blood, K.C. Allen Chan, Sze-Wan Yeung, Wing-Bong Lui, Timothy H. Rainer, and Y.M. Dennis Lo ( Department of Chemical Pathology and 2 Accident and Emergency Medicine Academic Unit, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, China; * address correspondence to this author at: Department of Chemic...
متن کاملاثرات گاز کلر بر سیکل سلولی و محتوی DNA گلبولهای سفید موش سوری The Effects of Chlorine on Cell Cycle and DNA Content of WBC in Mice
Background & Aim: Chlorine has been known as a mucus membranes and respiratory tract irritant. This gas can increase free radicals which cause cell damage. The aim of the present study was to measure DNA content and cell cycle in white blood cells after chronic chlorine poisoning. Materials & Methods: A clinical experimental study was carried out on 80 male mice(40 mice as sample and 40 as ...
متن کاملRole of Molecular Biomarkers in Colorectal Cancer
Colorectal cancer (CRC) is amongst the most widespread cancers and is a most common cause of cancer associated mortality universally. Since previous decades, it has been cleared that CRC develops owing to the buildup of a series of genetic and epigenetic changes in the normal colonic epithelium. Regardless of the current development in surgery and therapies, overall survival of end stage CRC pa...
متن کاملEffects of Major Epigenetic Factors on Systemic Lupus Erythematosus
The pathogenesis of systemic lupus erythematosus (SLE) is influenced by both genetic factors and epigenetic modifications; the latter is a result of exposure to various environmental factors. Epigenetic modifications affect gene expression and alter cellular functions without modifying the genomic sequences. CpG-DNA methylation, histone modifications, and miRNAs are the main epigenetic factors ...
متن کاملPreanalytical blood sample workup for cell‐free DNA analysis using Droplet Digital PCR for future molecular cancer diagnostics
In current molecular cancer diagnostics, using blood samples of cancer patients for the detection of genetic alterations in plasma (cell-free) circulating tumor DNA (ctDNA) is an emerging practice. Since ctDNA levels in blood are low, highly sensitive Droplet Digital PCR (ddPCR) can be used for detecting rare mutational targets. In order to perform ddPCR on blood samples, a standardized procedu...
متن کاملMolecular Effects of Atmospheric Pressure Plasma Jet on the Double-Stranded DNA
Introduction The aim of this study was toinvestigate the sterilization potential of atmospheric pressure plasma jet (APPJ) and interactions of this technology with double-stranded DNA using the polymerase chain reaction (PCR) and single-strand conformation polymorphism (SSCP) techniques. Materials and Methods The plasma jet was produced through a high voltage sinusoidal power supplyusing a mixt...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 51 4 شماره
صفحات -
تاریخ انتشار 2005