Lymphocyte surface and cytoplasmic changes associated with translational motility and spontaneous capping of Ig.
نویسندگان
چکیده
Murine B-lymphocytes during translatory motion undergo a series of changes with respect to their morphology and distribution of surface immunoglobulins (Ig). The sequence of events comprising these changes was followed by fluorescence microscopy and a correlated detection of surface features by scanning microscopy on exactly the same cell. A round, presumably non-motile lymphocyte exhibited a random distribution of Ig and its surface displayed evenly distributed microvilli. Formation of a ruffled edge at one pole, accompanied by a decreased fluorescence at this pole marked the initial events of lymphocyte motility. In the subsequent stages, the ruffled edge became progressively prominent and displayed a constriction at its base, while the microvilli were displaced to the opposite pole. Ig in such lymphocytes was localized at the trailing, microvilli-rich pole. Thin sections of motile lymphocytes revealed Ig, microtubules, microfilaments and coated vesicles as the characteristic features of the trailing end. These observations may have bearing on the mechanism of lymphocyte motility and spontaneous capping of Ig.
منابع مشابه
Brief Definitive Report Spontaneous Redistribution of Surface Immunoglobulin in the Motile B Lymphocyte* Materials and Methods the Journal of Experimental Medicine @bullet Volume
Most macromolecules are assumed to be distributed randomly on the cell surface, diffusing freely within the plane of the plasma membrane. However, upon interaction with ligands, surface proteins undergo an orderly redistribution and segregation from other macromolecules the phenomenon of capping (1). Capping of various molecules shares certain requirements: a cross-linking ligand producing the ...
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ورودعنوان ژورنال:
- Journal of cell science
دوره 39 شماره
صفحات -
تاریخ انتشار 1979