Versatile immunoassays based on isomagnetophoresis.

Streptavidin - A Versatile Binding Protein for Solid-Phase Immunoassays

Phage-borne peptidomimetics accelerate the development of polyclonal antibody-based heterologous immunoassays for the detection of pesticide metabolites.

Competitive immunoassays for the detection of small analytes, such as pesticides and their metabolites, use haptens that compete with the target compounds for binding to the antibody. This competing hapten can be either the same as the immunizing hapten (homologous assay) or structurally modified mimics of the immunizing hapten (heterologous assay). Polyclonal antibody-based heterologous immuno...

Advancing Label-free Microarray Immunoassays with Protein Capture of Antibodies A Demonstration by Oblique Incidence Reflectivity Difference (OI-RD) Ellipsometry

Oblique Incidence Reflectivity Difference (OI-RD), a system developed by Zhu et al, is a versatile, high-throughput, label-free method for probing reactions of biological molecules printed on standard substrates. However, its sensitivity range is currently not as developed as that of label-based techniques. The effectiveness of using recombinant fusion protein A/G to immobilize antibodies on a ...

Quantification of Human Chorionic Gonadotropin by Bovine Serum Albumin Nanoparticles

Background and Aims: Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs). Materials and methods: At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin ...

Brassica oleraceae, a versatile plant for green synthesis of silver nanoparticles

In the present paper, silver nanoparticles (AgNPs) were synthesized using the Brassica oleraceae fruit extract under the simple and eco-friendly conditions. The reaction between silver nitrate, as metal source, and aqueous extract of Brassica oleraceae fruit, as reductant agent, produced AgNPs in high yield. The formation of AgNPs was confirmed by means of UV-Vis spectroscopy and scanning elect...