Separation of Protein Crystals from Spores of Bacillus thuringiensis by Ludox Gradient Centrifugation.

Separation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain x-ray contrasting agents.

Spores and parasporal crystals of Bacillus thuringiensis can be separated at moderate centrifugation speeds (10,000 to 12,000 rpm) in gradients of Renografin or sodium diatrizoate.

Application of Membrane Separation Technology in Downstream Processing of Bacillus thuringiensis Biopesticide: A Review

Bacillus thuringiensis (Bt) has been extensively used in biopesticidal formulations due to its safe environmental and human health records. The widespread use of Bt is often challenged by production as well as formulation costs which are in direct contact with downstream processing, i.e. the separation/purification step. Downstream separation/purification efficacy governs the marketabi...

Purification of the protein crystal from Bacillus thuringiensis by zonal gradient centrifugation.

A method is described for the large-scale purification of the Bacillus thuringiensis protein crystal by zonal gradient centrifugation. NaBr gradients are employed in a Beckman J21-B centrifuge equipped with a JCF-Z rotor.

Laboratory Study of Microbial Insecticide Produced by the Bacteria Bacillus thuringiensis of Starch Factory Sewage for Biological Control of Larvae Culex pipiens

 Background & Objective: Epidemic of dangerous diseases caused by Culex pipiens’ bites prompted us to focus on these groups of insects. Today, the most successful biological insecticides are produced by the bacteria of the genus Bacillus, which in the production phase of spores, makes crystalline endotoxin protein. This protein can be activated upon entering the insect’s intestine in alkali...

Biphasic system for separation of spores and crystals of Bacillus thuringiensis.