, Unkeless , and Cohn 185
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چکیده
Mononuclear leukocytes are able to destroy tumor cells under a variety of circumstances. In few such instances is the biochemical basis of cytotoxicity understood. Recently, we demonstra ted that activated macrophages, s t imulated by phorbol myristate acetate (PMA), 1 secreted substantial amounts o f hydrogen peroxide (1). The release o f this oxidant from macrophages correlated with the extracellular lysis o f tumor cells (2). In fact, under the conditions tested, hydrogen peroxide was both necessary and sufficient for cytolysis by activated macrophages (3). The secretion by phagocytic leukocytes of toxic species o f reduced oxygen can be triggered not only pharmacologically, but also immunologically, such as by contact with ant ibodycoated particles or aggregated immunoglobul in (4-9). Therefore, we a t tempted to learn whether activated macrophages would lyse tumor cells when the tumor cells were coated with homologous antibody, and whether reactive metabolites of oxygen would play a role in this form of cytolysis. Tests of these hypotheses are reported below and in the accompanying paper (10).
منابع مشابه
NATURAL KILLER ACTIVITY IN THE PERITONEAL EXUDATES OF MICE INFECTED WITH LlSTERlA MONOCYTOGENES: CHARACTERIZATION OF THE NATURAL KILLER CELLS
30. Johnston, R. B.. C. A. Godzik. and 2. A. Cohn. 1978. Increased superoxide anion production by immunologically activated macrophages. J. Exp. Med. 148:115. 31. Nathan, C. F.. L. Brukner. G. Kaplan. J. Unkeless and 2 . Cohn. 1980. Role of activated macrophages in antibody-dependent lysis of tumor cells. J. Exp. Med. 152:183. 32. Nathan, C. F.. and 2. A. Cohn. 1980. Roleof oxygen-dependent mec...
متن کاملRole of activated macrophages in antibody-dependent lysis of tumor cells
Treatment of mice with Bacille Calmette-Guérin (BCG) or C parvum activates their peritoneal macrophages to release increased amounts of H2O2, and thereby to lyse extracellular tumor cells, in response to a pharmacologic agent, phorbol myristate acetate (PMA) (1-3). In the present study, the same bacterial vaccines activated peritoneal cells to become cytolytic to lymphoma cells sensitized with ...
متن کامل184 Activated Macrophages in Antibody
Mononuclear leukocytes are able to destroy tumor cells under a variety of circumstances. In few such instances is the biochemical basis of cytotoxicity understood. Recently, we demonstra ted that activated macrophages, s t imulated by phorbol myristate acetate (PMA), 1 secreted substantial amounts o f hydrogen peroxide (1). The release o f this oxidant from macrophages correlated with the extra...
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