SpecificImmunoassayof a-AmylaseIsoenzymesin HumanSerum
نویسندگان
چکیده
A monoclonalantibody(66C7) was preparedthat specifically binds human salivary amylase (EC 3.2.1.1); it cross reacts with human pancreatic amylase by less than 1%. Two procedures are described for determination of isoamylases in human serum with this antibody: an enzyme immunoassay for determining amylase of salivary origin, and a routine method in which this amylase is immunoprecipitated and the remaining(pancreatic)amylase activity is assayed. Results by the two methods correlate well.
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