Npgrj_nmeth_1136 37..39
نویسندگان
چکیده
Here we report the development of a gene-synthesis technology, circular assembly amplification. In this approach, we first constructed exonuclease-resistant circular DNA via simultaneous ligation of oligonucleotides. Exonucleaseand subsequent mismatch cleaving endonuclease–mediated degradation of the resulting ligation mixture eliminated error-rich products, thereby substantially improving gene-synthesis quality. We used this method to construct genes encoding a small thermostable DNA polymerase, a highly repetitive DNA sequence and large (44 kb) constructs.
منابع مشابه
Investigating thermal transport in isotope substituted nanomaterials using molecular simulations
......... ......................................................................................................... 23 References........ ........................................................................................................ 35 CHAPTER 4. DISSIMILAR HEAT CONDUCTION MECHANISMS IN ANALOGOUS 2D NANOMATERIALS WITH ISOTOPE SUBSTITUTION: GRAPHENE VERSUS SILICENE........................
متن کاملMonoclonal antibodies that disrupt poliovirus only at fever temperatures.
Three of thirty-six monoclonal antibodies were found to cause irreversible inactivation of type 1 poliovirus at 39 degrees C but not at 37 degrees C. Neutralization at 37 degrees C depended on aggregation and was reversible by acid-induced deaggregation; at 39 degrees C, the virions (N antigenic, 160S) were disrupted to empty capsids (H antigenic, 100S), and neutralization was irreversible. The...
متن کاملTiming of elective repeat cesarean delivery at term and maternal perioperative outcomes.
OBJECTIVE Elective repeat cesarean delivery at 37 or 38 weeks compared with 39 completed weeks of gestation is associated with adverse neonatal outcomes. We assessed whether delivery before 39 weeks is justifiable on the basis of decreased adverse maternal outcomes. METHODS We conducted a cohort study of women with live singleton pregnancies delivered by prelabor elective repeat cesarean deli...
متن کاملThe effects of methylamine osmolytes on the cytoskeleton microtubule assembly
16 Introduction 17 Materials and Methods 20 Results 23 Discussion 33 Acknowledgments 37 References 37 CHAPTER 3: TRIMTHYLAMINE N-OXIDE CAN PROMOTE 39 PHOSPHORYLATED TAU-TUBULIN ASSEMBLY Abstract 39 Introduction 41 Materials and Methods 4439 Introduction 41 Materials and Methods 44
متن کاملMeteorological Register for December, 1816
2 2| 35 W ditto 3 37 ditto O 4 2 39 NW ditto 5 1 29 5| 41 SW Rain 6 29 5 4 39 SW ditto 7 4 6 37 NW Fine and clear 8 5 6\ 39 W ditto 9 35 SE 10 5 2 41 W Rain 11 2 4 40 NW ditto > 12 2 28 40 SW Rain, G. of W. 13 28 9| 2 92 37 W Fine and clear 14 29 3 34 W Rain G. of W. 15 28 2 29 1^ 44 W ditto 16 29 3 4^ 33 W Fine and clear 17 2 2 48 W Rain ? 18 2\ 7 3S NW ditto 19 9 30 2 35 N Snow 20 30 3| 3| 33...
متن کامل