In-vitro Assessment of Antioxidant Activity of Dalbergia latifolia Barks Extract Against Free Radicals

Dalbergia latifolia Roxb. (Family: Fabaceae) is a traditional herb, contain latinone and dalcriodain flavonoid having excellence medicinal value. Antioxidants play an important role for protecting the human body against damage by reactive free radicals. Methods: The antioxidant activities and phenolic contents of the ethanolic extract of the bark of Dalbergia latifolia was evaluated using in vitro standard procedures for the determinations of total phenol, total flavonoid. Gallic acid and rutin equivalents were used for these parameters. The antioxidant activities of the bark extract of Dalbergia latifolia were determined by the 1,1-Diphenyl-2picrylhydrazyl (DPPH), nitrous oxide thiocyanate and reducing antioxidant property methods. Results: The total phenolic content was found to be 210±1.16 μg mL ; however the flavonoid content was 46±3.61 μg mL for 1 1 D. latifolia bark extract. DPPH, NO and thiocyanate percentage inhibition scavenging activity 92.10±1.10, 86.39±2.12 and 87.22±2.47 respectively. Conclusion: The antioxidant played an important role of protecting the human body against free radicals. The present data demonstrated that D. latifolia bark exerted significant antioxidant scavenging potential. Further, it requires identifying the exact phytoconstituents responsible for the activity.