Preservation and Maintenance of Bhk-21 Cell Line at Different Temperature Conditions
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چکیده
The present work was undertaken with an aim to standardize the preservation of BHK-21 cell lines at different temperature conditions viz., at 4 C , -20 C and in LN2 using appropriate freezing medium. The cell line after preservation was revived at 10 days and 40 days intervals. We observed that the cells maintained at 4 C and LN2 at -196 C were having the highest revival rates and within 24-36 hours we could see the complete revival of the cells.
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