Sulfhydryls-a new factor in frost resistance. II. Relation between sulfhydryls & relative resistance of 15 wheat varieties.
نویسندگان
چکیده
The wheat varieties were supplied by Dr. G. Aufhammer of the Institut fur Acker-und Pflanzenbau, at Freising-Weihenstephan, Germany. To obtain seedlings in the three-leaf stage preparatory to hardening, they were grown in flats of soil in the greenhouse undler prevailing temperatures during May to July. A photoperiod of 9 hours was used at all stages. The three-leaf seedlings were then hardened by transfer to a cold room at 3 C under either two 500-w incandescent bulbs (the radiations from which were cooled by a tray of water) or under ten 40-w fluorescent bulbs. The illumination at the leaf surface was 250 and 1,000 ft-c, respectively. To obtain seedlings in the coleoptile to one-leaf stage, the seeds were germinated in petri dishes of vermiculite covered with filter paper, for 5 days at 22 C and with the weak light prevailing in the laboratory. Two kinds of hardening were used for these: A, 4 days at -4 C in the dark, and B, 7 days at 2 C with a 9-hour day of weak light (70 ft-c). Frost-killing points were determined by suspending the seedlings in the freezing chamber, inoculating with snow, lowering the temperature 2 C/hour, and removing two plants to the cold room after each freezing temperature. After 24 hours at 3 C, they were then transferred to water at room temperature for a few days and the percent injury estimated. The frost killing point was the one producing more than 50 % killing of the leaves. The extracts for SH determinations were prepared in the cold room (3 C) as follows: 10 g fresh material were first cut into small pieces in 150 ml
منابع مشابه
Sulfhydryls-A New Factor in Frost Resistance. III. Relation of SH Increase During Hardening to Protein, Glutathione, & Glutathione Oxidizing Activity.
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ورودعنوان ژورنال:
- Plant physiology
دوره 36 5 شماره
صفحات -
تاریخ انتشار 1961