Binding of [14C]tuberactinomycin O to ribosomes from Mycobacterium smegmatis.
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چکیده
منابع مشابه
The effect of viomycin on elongation steps in cell-free polypeptide synthesis.
that VMinhibits protein synthesis was first shown in intact Mycobacterium aviurrP and it is a ribosomal inhibitor since VM resistance of certain mutants of Mycobacterium smegmatis and Mycobacterium tuberculosis can be attributed to an alteration of ribosomes4~7). The characteristics of VMbinding to ribosomes have been studied. The drug reduced the amounts of [3H]dihydrostreptomycin bound to rib...
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Viomycin-resistant strains isolated from Mycobacterium smegmatis demonstrated pleiotropic resistance to tuberactinomycin-N, capreomycin, streptomycin, and kanamycin as a result of mutational alteration of ribosomes, even though they were selected for resistance to a single antibiotic. The pleiotropic drug resistance of three mutants isolated by stepwise selection for resistance to viomycin was ...
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Protein translation is essential for all bacteria pathogens. It has also been a major focus of structural and functional studies and an important target of antibiotics. Here we report our attempts to biochemically reconstitute mycobacterial protein translation in vitro from purified components. This mycobacterial translation system consists of individually purified recombinant translation facto...
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Capreomycin and the structurally similar compound viomycin are cyclic peptide antibiotics which are particularly active against Mycobacterium tuberculosis, including multidrug resistant strains. Both antibiotics bind across the ribosomal interface involving 23S rRNA helix 69 (H69) and 16S rRNA helix 44 (h44). The binding site of tuberactinomycins in h44 partially overlaps with that of aminoglyc...
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Lividomycin inhibited incorporation of acetate-114C , glutamic acid-1-14C, leucine-1-14C, and glycine1-14C into protein of Mycobacterium smegmatis (strain Jucho). Incorporation of acetate-1-14C and glycine1-14C into nucleic acids was also inhibited, but incorporation of these compounds into lipids was not inhibited in the presence of lividomycin. Incorporation of uridine-2-14C into nucleic acid...
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ورودعنوان ژورنال:
- The Journal of antibiotics
دوره 38 2 شماره
صفحات -
تاریخ انتشار 1985