Sequence of a giardin subunit cDNA from Giardia lamblia.
نویسندگان
چکیده
The filamentous protein giardin is structurally associated with microtubules in Giardia lamblia. We present the sequence of one giardin chain (beta-giardin) corresponding to band 14B of Giardia cytoskeleton proteins (1). A Giardia cDNA library cloned and expressed in lamda gt11 was screened with antibodies monospecific to giardin (2). One positive phage clone (SR7) selected for sequencing contained an insert of 839 bp encoding the complete giardin polypeptide of 29.4 kD. The amino acid sequence of 259 residues is predicted to be alpha-helical (3). An unusual polyadenylation signal (AGTAAA) is present in the 3 1 non-coding region of the cDNA sequence.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 16 14B شماره
صفحات -
تاریخ انتشار 1988