FTIR Spectroscopy Revealing Light-Dependent Refolding of the Conserved Tongue Region of Bacteriophytochrome
نویسندگان
چکیده
Bacteriophytochromes (BphPs) constitute a class of photosensory proteins that toggle between Pr and Pfr functional states through absorption of red and far-red light. The photosensory core of BphPs is composed of PAS, GAF, and PHY domains. Here, we apply FTIR spectroscopy to investigate changes in the secondary structure of Rhodopseudomonas palustris BphP2 (RpBphP2) upon Pr to Pfr photoconversion. Our results indicate conversion from a β-sheet to an α-helical element in the so-called tongue region of the PHY domain, consistent with recent X-ray structures of Deinococcus radiodurans DrBphP in dark and light states (Takala H.; et al. Nature2014, 5, 245-248). A conserved Asp in the GAF domain that noncovalently connects with the PHY domain and a conserved Pro in the tongue region of the PHY domain are essential for the β-sheet-to-α-helix conversion.
منابع مشابه
Light-induced structural changes in a monomeric bacteriophytochrome
Phytochromes sense red light in plants and various microorganism. Light absorption causes structural changes within the protein, which alter its biochemical activity. Bacterial phytochromes are dimeric proteins, but the functional relevance of this arrangement remains unclear. Here, we use time-resolved X-ray scattering to reveal the solution structural change of a monomeric variant of the phot...
متن کاملThe effect of incoherent ultraviolet light on the renal capsule tissue using dynamic mechanical analysis and FTIR spectroscopy
متن کامل
Light and electron microscopic study of the tongue in the White-eared bulbul (Pycnonotus leucotis)
The aim of the present study was to investigate the light and electron microscopic structure of the tongue in White-eared bulbul (Pycnonotus leucotis). Six adult White-eared bulbul (3 males and 3 females) in Shahrekord district were used in the investigation. Samples of the apex, body and root of the tongue were prepared routinely for light and electron microscopic study. Results showed that th...
متن کاملTemperature-jump-induced refolding of ribonuclease A: a time-resolved FTIR spectroscopic study.
FTIR difference spectroscopy has been used for the first time to investigate the kinetics of secondary structure formation during refolding. The refolding process of ribonuclease A (RNase A) as a model system was induced by applying a temperature-jump of 60 degrees. The temperature-jump was triggered by rapidly injecting a small volume of the thermally unfolded protein solution at 80 degrees C ...
متن کاملLight and scanning electron microscopic study of the lingual structure in the budgerigar (Melopsittacus undulatus)
BACKGROUND: The tongue, which plays a very important role in food intake by vertebrates, exhibits significant morphological variations that appear to represent adaptation to the current environmental conditions of each respective habitat. OBJECTIVES: The aim of the present investigation was to investigate lingual structure in adult budgerigar. METHODS: Tongues of 12 adult budgerigars were used ...
متن کامل