Js-lactamase and 8-lactam Antibiotics Resistance in Acinetobacter Anitratum (syn.: A. Calcoaceticus)
نویسندگان
چکیده
The cephalosporin fl-lactamase (cephalosporinase) produced by Acinetobacter was studied. The enzyme was partially purified by means of column chromatography and its properties were investigated. The enzyme was induced by benzylpenicillin, 6-aminopenicillanic acid and cephaloridine. Its molecular weight is 30,000, its optimal temperature 40°C, and its optimal pH 7.25-.7.50. Substrate specificity studies using various cephalosporins and penicillins, showed that the enzyme functioned as a cephalosporinase rather than penicillinase.
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