Conferred Synthetase Promoter of Arabidopsisby the S - Adenosyl - L - Methionine thaliana in Transgenic Poplar
نویسنده
چکیده
adenosyl-L-methionine synthetase (SAM-S) Psam-1 confers expression preferentially in the yascular tissue. In search for promoters that driye expression in particular ce]ls of the lignifying tisslles i" trees, we haye analyzed the expression pattern conferred by the Psam-1 promoter in transgenic pop]ar. Histochemica] analyses demonstrated fi glucuronidase (GUS} actiyity mainly in phloem and cortex tissue throughout the plftnt, and in root tips. Fluorimetric assays showed high GUS activity in the tissues outside (phloem, cortex and cork) compared to those inside (xylem and pith) of the cambial layer. In contrast, the endogenous SAM-S actiyity was high in tissues inside and ]ow in tissues
منابع مشابه
Tissue-specific expression conferred by the S-adenosyl-L-methionine synthetase promoter of Arabidopsis thaliana in transgenic poplar.
In Arabidopsis the promoter of the gene encoding S-adenosyl-L-methionine synthetase (SAM-S) Psam-1 confers expression preferentially in the vascular tissue. In search for promoters that drive expression in particular cells of the lignifying tissues in trees, we have analyzed the expression pattern conferred by the Psam-1 promoter in transgenic poplar. Histochemical analyses demonstrated beta-gl...
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