Preparation of pleuropneumonia-like organisms for microscopic study.

I This investigation was supported in part by research grants (E-2764 and E-2765) from the National Institute of Allergy and Infectious Diseases, U. S. Public Health Service. 2 Special Research Fellow, U. S. Public Health Service. with differential staining would aid in their identification. There are two basic methods currently being used for the preparaticn of PPLO colonies for microscopic study. One methcd uses a modified agar fixation technique (Klieneberger and Smiles, J. Hyg., 42, 110, 1942) and the other a direct, stained agar technique (Dienes, J. Infectious Diseases, 65, 24, 1939). Both methods have been used and reported by other investigators to have