Accurate and sensitive mutation detection and quantitation using TaqMan Mutation Detection Assays for disease research
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چکیده
addressed the feasibility of obtaining sensitive, accurate, and reproducible mutation detection results in synthetic templates, cell lines, and FFPE tissue samples using TaqMan Mutation Detection Assays. Analytical performance assessed with synthetic templates and genomic DNA (gDNA) extracted from cell lines or model FFPE cell lines indicates that TaqMan Mutation Detection Assays have a minimum of 0.1% sensitivity and can detect fewer than 10 copies of mutant DNA sequence in a background of wild type gDNA. Our data indicate that TaqMan Mutation Detection Assays can accurately detect mutation status and quantify the percent mutation with a relative standard deviation (CV) <20%. Additionally, benchmarking to an established qPCR assay technology for mutation detection demonstrates that the TaqMan Mutation Detection Assays have a wider detection range and superior limit of detection. Accurate and sensitive mutation detection and quantitation using TaqMan Mutation Detection Assays for disease research APPLICATION NOTE TaqMan Mutation Detection Assays
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