Solid-phase microextraction method for the determination of volatile compounds associated to oxidation of fish muscle.
نویسندگان
چکیده
A procedure for the determination of volatile compounds derived from lipid oxidation of fish muscle samples is presented. Analytes are concentrated on a solid-phase microextraction fiber employed in the headspace mode (HS-SPME), and selectively determined using gas chromatography in combination with mass spectrometry (GC-MS). The influence of several parameters on the efficiency of microextraction such as type of fiber, volume of sample, time, temperature, salting-out effect and stirring was systematically investigated. A saline extraction of fish muscle followed by incubation on a Carboxen-polydimethylsiloxane fiber during 30min at 60 degrees C gave the most effective and accurate extraction of the analytes. Quantification of them was performed by MS in the selected ion monitoring mode and by the internal standard method. Satisfactory linearity, repeatability and quantification limits were achieved under these conditions. The method was applied to the determination of the volatile compounds associated to oxidation of Atlantic horse mackerel (Trachurus trachurus) minced muscle and excellent correlations were obtained with chemical indexes for monitoring lipid oxidation as peroxide value and thiobarbituric acid reactive substances. This combined technique is fast, simple, sensitive, inexpensive and useful to monitor target compounds associated to fish rancidity as 1-penten-3-ol, 2,3-pentanedione or 1-octen-3-ol.
منابع مشابه
Recent Advances in Microextraction Methods for Sampling and Analysis of Volatile Organic Compounds in Air: A Review
Human exposures to volatile organic compounds (VOCs) are associated with a wide range of health problems. Due to these adverse effects of VOCs on the human health, determination of trace levels of VOCs is very important for accurate assessment of indoor and outdoor exposure. Solid phase microextraction (SPME), needle trap device (NTD) and hollow fiber- liquid phase microextraction (HF-LPME) are...
متن کاملZnO polythiophene SBA-15 nanoparticles as a solid-phase microextraction fiber for fast determination essential oils of Matricaria chamomilla
The objective of this study is determination the volatile compounds of Matricaria chamomilla of an inorganic–organic hybrid material based on ZnO nanoparticles anchored to a composite made from polythiophene and hexagonally ordered silica (ZnO/PT/SBA-15) as a solid-phase fiber microextraction (SPME). Microextraction techniques with nanoparticles ZnO/PT/SBA-15 resulted in a more efficient analyt...
متن کاملUsing microwave distillation-solid-phase microextraction--gas chromatography--mass spectrometry for analyzing fish tissue.
A technique for the analysis of the volatile compounds from fish tissue employing microwave distillation-solid-phase microextraction-gas chromatography-mass spectrometry is described. A qualitative listing of 174 compounds observed in the headspace is given, and a quantitative method for the determination of the off-flavor contaminants (2-methylisoborneol and geosmin) is presented. Borneol and ...
متن کاملSpectrophotometric determination of total phenol in fish muscle after in- situ derivatization and reverse phase dispersive liquid- liquid microextraction
Abstract Reverse phase dispersive liquid- liquid microextraction (RP- DLLME) coupled with spectrophotometry is introduced for the detection of total phenol in the muscle tissue of fishes, such as Sphyraena genie, Otolithes ruber, Rastrelliger kangurta, Lutjanus johnii and Barbus Subquin Cunciatus. Phenols in fish tissues were extracted by ultrasonic bath and derivatization with 4- aminoant...
متن کاملOptimizing Sourdough Processing for the Production of ɣ-nonalactone (Fruity Aroma) by Lactobacillus sanfranciscensis Using Headspace Solid-Phase Microextraction Method
Background and Objectives: Sourdough processing is a well-known method to improve food flavor. One of the most important lactic acid bacteria isolated from sourdoughs is Lactobacillus sanfranciscensis. Because of the bacterial ability for acidification and liberation of the volatile compound precursors, it can strongly affect dough rheology and aromatic properties. In this study, the best condi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of chromatography. A
دوره 1192 1 شماره
صفحات -
تاریخ انتشار 2008