Single-tube single-enzyme reverse transcriptase PCR assay for detection of bovine viral diarrhea virus in pooled bovine serum.
نویسندگان
چکیده
A reverse transcriptase PCR (RT-PCR) was developed for use as a diagnostic screening test for the detection of bovine viral diarrhea virus (BVDV) in pooled bovine serum samples. Individual serum samples from 60 dairy cattle herds located in Pennsylvania were evaluated by the microplate virus isolation method, and pooled sera were analyzed by RT-PCR. RT-PCR was sensitive and specific and detected a single viremic serum sample in up to 100 pooled serum samples. RT-PCR analysis of pooled sera provides a rapid and cost-effective method for the screening of cattle herds for the presence of animals persistently infected with BVDV.
منابع مشابه
Bovine Serum Bovine Viral Diarrhea Virus in Pooled Transcriptase PCR Assay for Detection of Single-Tube Single-Enzyme Reverse
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 39 1 شماره
صفحات -
تاریخ انتشار 2001