Differences between antibodies against the dense sediment and microsome fractions of the N-2-fluorenylacetamide-induced rat hepatoma.

Received for publication January 22, 1965. were fixed after injection were determined by the use of †̃25I-labeledantibody and radioautography and were found to be mainly in connective tissues and occasionally on sinusoid walls (5). The inicrosome fraction of cells is derived from the endoplasmic reticulum, which has been claimed to be contiguous to cell surface. Moreover, Wallach and Hager have shown in the case of Erhlich ascites cells that this fraction contains antigens associated with cell surface (8). Since the localization of antibodies on cells would occur most effectively on cell surface, studies were under taken to examine antibodies against the microsome fraction of rat hepatoma. Our previous report clearly indicated that antibodies against hepatoma microsomes did localize in the hepatomas and in normal livers when injected (4).