Rapid isolation of differentially expressed cDNAs from near isogenic lines of mungbean
نویسندگان
چکیده
We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.11.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones were probably full-length cDNAs.
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