Serological comparison of spherules and arthrospores of Coccidioides immitis.
نویسندگان
چکیده
Spherule and arthrospore cellular preparations were sonic-treated and separated into their respective supernatant and sediment components. Complement-fixation tests with antispherule and antiarthrospore pooled rabbit sera revealed that the soluble antigens exhibited more serological activity than the sediment preparations. After autoclaving, an arthrospore cellular antigen exhibited increased activity with either antisera, whereas autoclaved spherules exhibited increased activity only with antispherule serum. Complement-fixation tests with coccicioidin and spherule culture supernatant preparations revealed quantitative or qualitative differences in antigenic determinants between these two morphological phases of Coccidioides immitis.
منابع مشابه
Serological comparison of the three morphological phases of Coccidioides immitis by the agar gel diffusion method.
Hyperimmune sera against spherules and against arthrospores of Coccidioides immitis were prepared by inoculation of rabbits. The antibody content of these sera was studied by the agar gel diffusion method. It was observed that antispherule pooled sera formed multiple precipitin bands with extracts of spherules and of arthrospores. The antiarthrospore pooled serum, however, failed to precipitate...
متن کاملStudies on coccidioides immitis. II. Physiological studies on in vitro spherulation.
Brooks, Lula D. (Arizona State University, Tempe) and William T. Northey. Studies on Coccidioides immitis. II. Physiological studies on in vitro spherulation. J. Bacteriol. 85:12-15. 1963.-Studies on the amino acids assayed in Edamine revealed that the amino acids phenylalanine, tyrosine, and tryptophan, in addition to the amino acid derivatives dihydroxyphenylalanine (DOPA), epinephrine, tyram...
متن کاملEffect of physico-chemical environment of spherulation of Coccidioides immitis in a chemically defined medium.
Coccidioides immitis undergoes a completely different type of growth in vivo from that in vitro. The parasitic form, or spherule, appears in animal tissue as a non-budding, spherical structure 20 to 80 ,u in diameter with a thick, refractile, double wall, and is filled witlh numerous small endospores 2 to 5 ,u in diameter. The saprophytic form appears in laboratory media as mycelium with branch...
متن کاملIn vitro production of the spherule phase of Coccidioides immitis.
The in vitro growth of the spherule (sporangium) phase of Coccidioides immitis has posed a problem of prime interest in the study of the disease coccidioidomycosis. The fungus is dimorphic in that it has a cultural, or saprophytic, phase in which growth consists of mycelium and arthrospores, and a tissue or parasitic phase consisting of thick-walled nonbudding spherules (sporangia) varying in s...
متن کاملDelayed dermal hypersensitivity in mice to spherule and mycelial extracts of Coccidioides immitis.
Kong, Yi-chi M. (University of California, Berkeley), D. C. Savage, and Leighton N. L. Kong. Delayed dermal hypersensitivity in mice to spherule and mycelial extracts of Coccidioides immitis. J. Bacteriol. 91:876-883. 1966.-A delayed hypersensitivity reaction to spherule and mycelial extracts of Coccidioides immitis was elicited in the footpads of mice vaccinated with killed spherules. Emulsifi...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 94 5 شماره
صفحات -
تاریخ انتشار 1967