Detection of Tetanus Antitoxin Using Eu-Labeled Anti-Human Immunoglobulin G Monoclonal Antibodies in a Time- Resolved Fluorescence Immunoassay
نویسندگان
چکیده
Tetanus antitoxin in human sera was detected with solid-phase immunoassays in microtitration modules coated with tetanus toxoid by using Eu+ -labeled anti-human monoclonal antibodies on the basis of an exactly calibrated antibody standard. The use of a time-resolved fluorescence immunoassay (TR-FIA) significantly improved the quantitative detection of tetanus antitoxin over that of the enzyme-linked immunosorbent assay (ELISA) technique because of its high sensitivity and its wide measurement range, detecting antibody levels between 0.001 and 12.5 IU/ml with a single serum dilution of 1:100. For the same purpose, two different serum dilutions (1:100 and 1:1,000) were needed in the ELISA technique.
منابع مشابه
Detection of tetanus antitoxin using Eu(3+)-labeled anti-human immunoglobulin G monoclonal antibodies in a time-resolved fluorescence immunoassay.
Tetanus antitoxin in human sera was detected with solid-phase immunoassays in microtitration modules coated with tetanus toxoid by using Eu(3+)-labeled anti-human monoclonal antibodies on the basis of an exactly calibrated antibody standard. The use of a time-resolved fluorescence immunoassay (TR-FIA) significantly improved the quantitative detection of tetanus antitoxin over that of the enzyme...
متن کاملElectrochemical assay of anti-tetanus toxoid monoclonal antibody by silver enhancement of gold nanoparticles at carbon nanotubes modified glassy carbon electrode
Tetanus is caused by the toxin secreted by Clostridium tetani. Due to the rapid infection with this bacterium, it is so important to investigate the tetanus immunity of people. Therefore, electrochemical biosensors, as one of the most effective tools in this regard, have demanded characteristics such as being fast, simple, cost-effective and portable. However, their detection sensitivity is not...
متن کاملHighly Sensitive FRET-Based Fluorescence Immunoassay for Detecting of Aflatoxin B1 Using Magnetic/Silica Core-Shell as a Signal Intensifier
Background: Recently, some new nanobiosensors using different nanoparticles or microarray systems for detection of mycotoxins have been designed . However, rapid, sensitive and early detection of aflatoxicosis would be very helpful to distinguish high-risk persons. Objectives: We report a highly sensitive competitive immunoassay using magnetic/silica core shell as a signal intensifier for the d...
متن کاملMonitoring ovarian function by the simultaneous time-resolved fluorescence immunoassay of two urinary steroid metabolites.
We report the development of a novel time-resolved fluorescence immunoassay utilizing two different assay strategies for the simultaneous measurement of estrone-3-glucuronide (EG) and pregnanediol-3alpha-glucuronide (PG) in samples of early morning urine (EMU). The method for the measurement of EG involves the use of a labeled anti-idiotype as a surrogate antigen, whereas the other method (for ...
متن کاملAPPLICATION OF DOT IMMUNOASSAY (DIA) FOR DETECTION AND TITRATION OF MEASLES VIRUS ANTIBODIES
Measles is one of the most contagious human diseases. Although mass vaccination programs have reduced the incidence of this disease, measles is still an important cause of morbidity and mortality among children in developing countries. Therefore the use of sensitive techniques to evaluate vaccine efficacy and level of immunity among members of susceptible communities is crucial. Serum neut...
متن کامل