Method and System for Diagnosis of Lawsonia Intracellularis
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منابع مشابه
Association between faecal load of lawsonia intracellularis and pathological findings of proliferative enteropathy in pigs with diarrhoea
BACKGROUND The study was designed to investigate correlation between histological findings of Lawsonia intracellularis in porcine cases of diarrhoea and the quantitative detection of Lawsonia intracellularis in faeces. A total of 156 pigs (10 to 70 days post weaning) with diarrhoea were randomly selected from 20 herds: The pigs were subjected to necropsy, histopathology, immunohistochemistry an...
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The obligate intracellular bacterium Lawsonia intracellularis causes enteritis and poor growth in weaned pigs. Cultivation is difficult and diagnosis ante mortem is mainly based on techniques such as polymerase chain reaction. However, false negative results caused by the presence of PCR-inhibitory factors constitute a problem. This study aimed to develop and evaluate a new technique, flotation...
متن کاملDetection of Lawsonia intracellularis using immunomagnetic beads and ATP bioluminescence.
Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in various animals. The detection of L. intracellularis in clinical and environmental samples is necessary for the diagnosis of infection and epidemiological investigations. For the detection of L. intracellularis in fecal samples, we have developed an immunological method using immu...
متن کاملLawsonia Intracellularis: A Constant Challenge to Modern Swine Production
Lawsonia intracellularis (LI) is an obligate intracellular proteobacteria that causes porcine proliferative enteritis. The disease affects mainly piglets and has an important role in economic losses in the swine breeding chain, chiefly because it is usually hardly noticed swine herds. Being a disease with cosmopolitan distribution it triggers reduction in average daily gain of weight and feed c...
متن کاملApplication of real-time PCR for detection of Lawsonia intracellularis and Brachyspira hyodysenteriae in fecal samples from pigs.
The aim of the study was to develop and validate real-time PCR method for the quantification of Lawsonia intracellularis and Brachyspira hyodysenteriae in porcine feces. Before the optimization process was performed two different extraction methods were compared to select the more efficient one. Based on the results achieved at this stage the boiling procedure was rejected and a commercially av...
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تاریخ انتشار 2017