Microfiltration Device for Continuous, Label-free Bacteria Separation from Whole Blood for Sepsis Treatment
نویسندگان
چکیده
This work presents the design, fabrication, and testing of a cross-flow filtration microdevice, for the continuous extraction of bacteria from a whole blood sample for the treatment of sepsis and bacteremia. The system consists of a two-compartment microdevice with two aligned sets of PDMS microchannels, separated by a porous polycarbonate (PCTE) membrane with 2 m pores. When a whole blood sample flows through the channels on one side of the membrane (Reservoir channels), the larger blood cells are sterically excluded from passing through the membrane while the smaller bacteria are removed by traversing the membrane into the filtration channels.
منابع مشابه
Elasto-inertial microfluidics for bacteria separation from whole blood for sepsis diagnostics
BACKGROUND Bloodstream infections (BSI) remain a major challenge with high mortality rate, with an incidence that is increasing worldwide. Early treatment with appropriate therapy can reduce BSI-related morbidity and mortality. However, despite recent progress in molecular based assays, complex sample preparation steps have become critical roadblock for a greater expansion of molecular assays. ...
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