Highly multiplexed assays for measuring polymorphisms on the Y-chromosome
نویسندگان
چکیده
A multiplex PCR assay capable of amplifying 20 Y-chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the ‘‘extended haplotype’’ used in Europe (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, and YCAII) plus additional polymorphic Y STRs (DYS437, DYS438, DYS439, DYS447, DYS448, DYS388, DYS426, GATA A7.1, and GATA H4). Single nucleotide polymorphism (SNP) assays that may be rapidly analyzed with time-of-flight mass spectrometry have also been developed using Y-chromosome bi-allelic markers. With improved multiplexes and techniques for more rapid data collection such as mass spectrometry, Y-chromosome haplotypes may be more quickly generated for DNA databases and population studies and forensic cases may be solved more efficiently. D 2003 Elsevier Science B.V. All rights reserved.
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