Biphasic system for growing bacteria in concentrated culture.

نویسندگان

  • E A TYRRELL
  • R E MACDONALD
  • P GERHARDT
چکیده

The concentration of masses of pathogenic bacteria can be simplified by growing them in a confined environment with diffusional access to a reservoir of nutrients. Culturing them on an agar or membrane surface accomplishes this; however, the total cell yield often is low, the cells probably are not physiologically uniform, and difficulty may occur in dislodging them. The concentration of cells or their products also may be obtained in a liquid medium by using a cellophane sac to separate the culture from a large nutrient supply (Hestrin et al., 1943; Gorelick et al., 1951; Vinet and Fredette, 1951). A combination of these principles led us 'to the idea of concentrating bacteria in a biphasic growth system that consists simply of a layer of solid nutrient medium overlaid with a small volume of nutrient broth. Hestrin et al. (1943) briefly mentioned the use of aqueous sucrose over a nutrient agar for the production of bacterial levansucrase, and W. C. Haynes stated in a personal communication that he has grown staphylococci in a liquid-solid system using charcoal agar. In this investigation of a biphasic growth system, we have obtained bacterial populations of up to 6 X 101.1 viable cells per ml, representing density increases of 2to 30-fold over broth controls, without sacrificing total yield.

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عنوان ژورنال:
  • Journal of bacteriology

دوره 75 1  شماره 

صفحات  -

تاریخ انتشار 1958