Somatic embryogenesis and plant regeneration in Soybean [Glycine max (L.) Merr.]
نویسندگان
چکیده
Establishment of efficient protocol for the induction of somatic embryogenesis and plant regeneration from embryonic axes explants was achieved in Glycine max (L.) Merr. Highest frequency (92.9%) of embryogenic callus formation was obtained from embryonic axes explants of cv. CO-1 on MS media containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyl adenine (BA). Fast growing, yellowish nodular callus lines containing somatic embryos were established on initiation medium supplemented with 180.8 μM 2,4-D and 2.22 μM BA, respectively. Embryo development and maturation was achieved on MS medium supplemented with 2.26 μM 2,4-D and 8.88 μM BA. The well formed embryos germinated into complete plantlets on MS medium containing 8.88 μM BA and 1.34 μM NAA (α-Naphthalene acetic acid). The regenerated plants were first transferred to plastic cups and later into pots for maturation. Regenerated plants did not exhibit observable morphological alterations.
منابع مشابه
بررسی تأثیر برخی عوامل مؤثر بر جنینزایی و بلوغ جنینهای رویشی در سویا (Glycine max)
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