Sulfide Production from Cysteine by Desulfovibrio desulfuricans.
نویسنده
چکیده
Two rumen nitrate-reducing isolates of Desulfovibrio desulfuricans were found to hydrolyze cysteine with the production of sulfide and pyruvate. When cultured on agar medium containing yeast extract with nitrate as the primary electron acceptor and ferrous chloride as the indicator, blackening of colonies occurred. The blackening of colonies appeared sooner and was more intense when either cysteine or sulfate was added to the culture medium with nitrate present.
منابع مشابه
Desulfovibrio of the sheep rumen.
A sulfate-reducing bacterium has been isolated in pure culture from sheep rumen contents. Its properties agree in all respects tested with those ascribed to Desulfovibrio desulfuricans. The populations observed (about 10(8)/ml) are sufficient to account for published rates of ruminal sulfide production.
متن کاملPurification of acetokinase from Desulfovibrio desulfuricans.
The formation of acetyl phosphate and adenosine diphosphate (ADP) from adenosine triphosphate (ATP) and acetate was first recognized by F. Lipmann (J. Biol. Chem. 155:55, 1944) in extracts of Lactobacillus delbrueckii and later by E. R. Stadtman and H. A. Barker (J. Biol. Chem. 184:769, 1950) in Clostridium kluyveri. I. C. Rose et al. (J. Biol. Chem. 211:737, 1954) purified the acetokinase of E...
متن کاملReduction of uranium by Desulfovibrio desulfuricans.
The possibility that sulfate-reducing microorganisms contribute to U(VI) reduction in sedimentary environments was investigated. U(VI) was reduced to U(IV) when washed cells of sulfate-grown Desulfovibrio desulfuricans were suspended in a bicarbonate buffer with lactate or H2 as the electron donor. There was no U(VI) reduction in the absence of an electron donor or when the cells were killed by...
متن کاملAmino acid sequence of the [4Fe-4S] ferredoxin isolated from Desulfovibrio desulfuricans Norway.
The complete amino acid sequence of the [4Fe-4S] ferredoxin from Desulfovibrio desulfuricans Norway was determined by repetitive Edman degradation of the whole protein and peptides derived from tryptic digestion. The protein has 59 residues. Four of the six cysteine residues are involved in the binding of the [4Fe-4S] cluster in the same arrangement as in clostridial ferredoxins. This sequence ...
متن کاملEvidence for oxidative phosphorylation during the reduction of sulfate with hydrogen by Desulfovibrio desulfuricans.
Desulfovitio d&dfUTiCam is an obligately anaerobic organism that can bc grown heterotrophically with sulfate and an organic electron donor or autotrophically with sulfate, hydrogen, and CO* (1). The presence of sulfate is obligatory for growth both with hydrogen and organic electron donors, except in the case of pyruvate (2). During growth, large amounts of sulfide are produced from the sulfate...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 39 2 شماره
صفحات -
تاریخ انتشار 1980