Wood Species and Culture Age Affect Zone Line Production of Xylaria polymorpha

Three pure cultures of Xylaria polymorpha were isolated from fruiting bodies at yearly intervals over two years and maintained on 2% malt agar plates at room temperature. Immediately after isolation of the third culture, the cultures were inoculated onto sugar maple (Acer saccharum), aspen (Populus tremuloides), birch (Betula alleghaniensis), and basswood (Tilia americana) 14 mm cubes and incubated for 10 weeks in jars containing vermiculite. More zone lines were produced on aspen and sugar maple than on yellow birch or basswood. Increasing culture age generally caused a decrease in zone line production; however the effect was only statistically significant in sugar maple. The results indicate that aspen is preferable for zone line production with X. polymorpha, as both external and internal zone lines occur on this wood species, and zone line production remains high despite the age of the culture.