Light Sheet Module Applied in 3d Live Cell Microscopy
نویسندگان
چکیده
Innovative fiber-coupled add-ons for light sheet-based fluorescence microscopy (LSFM) with commercial inverted microscopes were described previously [1]. The light sheet is generated either by an achromatic cylindrical lens or by a spherical mirror with astigmatic distortion. Presently the “lens system” is further validated and applied to various samples including 3D multicellular spheroids and small organisms. Samples are commonly located in rectangular glass capillaries or in rotatable cylindrical capillaries optically coupled to the rectangular capillary [2].
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Miniaturized modules for light sheet microscopy with low chromatic aberration.
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