A Molecular and Immunological Investigation of Cellular Responses to Dengue Virus: Identification of Potentially Upregulated Host Genes and the Construction of a Vaccinia Virus Expressing the Dengue 1 Hawaii NS3 Protein
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چکیده
........ ............................................................................................... ii List of Figures and Tables ............................................................................. iii Acknowledgements ....................................................................................... iv General Background...................................................................................... 1 Purpose of Thesis .......................................................................................... 5 PART 1: Identification of Potentially Upregulated Genes of K562 Cells due to Dengue Type 2 Infection Background ....................................................................................... 6 Materials and Methods ...................................................................... 8 Results ............................................................................................... 12 Discussion ......................................................................................... 18 PART 2: Construction and Use of a Recombinant Vaccinia Virus Expressing the Dengue Type 1 (Hawaii) NS3 Protein in Immunological Studies Background ....................................................................................... 24 Materials and Methods ...................................................................... 28 Results ............................................................................................... 36 Discussion ......................................................................................... 48 Bibliography.................................................................................................. 51
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