Early enzyme synthesis and its control in E. coli infected with some amber mutants of bacteriophage T4.
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چکیده
3Wood, H. G., and R. Stjernholm, these PROCEEDINGS, 47, 289 (1961). 4Stjernholm, R., and H. G. Wood, Fed. Proc., 20, 235 (1961). 5 Beck, W. S., and S. Ochoa, J. Biol. Chem., 232, 931 (1958). 6 Smith, R. M., and K. J. Monty, Biochem. Biophys. Research Commun., 1, 105 (1959); Stadtman, E. R., P. Overath, H. Eggerer, and F. Lynen, Biochem. Biophys. Research Commun., 2, 1 (1960); Lengyel, P., R. Mazumder, and S. Ochoa, these PROCEEDINGS, 46, 1312 (1960); Gurnani, S., S. P. Mistry, and B. C. Johnson, Biochim. et Biophys. Acta, 38, 187 (1960); Stern, J. R., and D. L. Friedman, Biochem. Biophys. Research Commun., 2, 82 (1960). 7 Munch-Petersen, A., and H. A. Barker, J. Biol. Chem., 230, 649 (1958). 8 Eggerer, H., P. Overath, F. Lynen, and E. R. Stadtman, J. Am. Chem. Soc., 82, 2643 (1960). 9 Phares, E. F., M. V. Long, and S. F. Carson, Bact. Proc., 61, 184 (1961). 10 Kosower, E. M., personal communication. 11 Beck, W. S., M. Flavin, and S. Ochoa, J. Biol. Chem., 229, 997 (1957).
منابع مشابه
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 48 شماره
صفحات -
تاریخ انتشار 1962