The early pathogenesis of foot-and-mouth disease in pigs infected by contact: a quantitative time-course study using TaqMan RT-PCR.

Rapid and accurate diagnosis of Foot-and-mouth disease virus by Real-time PCR in field samples

During 2010-2011, Real-time PCR procedure was used to detecting FMDV RNA on 147 epithelium samples from the field. In this survey, for Real-time PCR from 3D gene segment as conserve region selected for tracking all of seven serotypes FMDV. The assay detected the viral RNA in all serotypes of FMDV. The rRT-PCR specifically detected FMD virus in sample with greater sensitivity than our convention...

Evaluation of cytokine mRNA expression in vaccinated guinea pigs with foot-and-mouth disease type O inactivated vaccine

Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs withFMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyz...

Evaluation of Humoral Immune Response to Foot-and-Mouth Disease Vaccination in Experimentally Infected Guinea Pigs with Trypanosoma evansi

Foot-and-mouth disease virus infection in young lambs: pathogenesis and tissue tropism.

Foot-and-mouth disease (FMD) in adult sheep usually causes milder clinical signs than in cattle or pigs, and is often subtle enough to go undiagnosed. In contrast, FMD in lambs has been reported to cause high mortality during field outbreaks. In order to investigate the pathogenesis of FMD in lambs, two groups, aged 10-14 days, were infected with foot-and-mouth disease virus (FMDV) type O UKG. ...

Loop-mediated isothermal amplification (RT-LAMP): a new approach for the detection of foot-and-mouth disease virus and its sero-types in Pakistan

Successful disease management requires a rapid and sensitive diagnosis method that can recognize early infection even before the manifestation of its clinical signs. The only available field diagnostic tests for foot-and-mouth disease (FMD) are lateral flow devices, commonly known as chromatographic strips. Low sensitivity and inability to detect FMD virus (FMDV) at the serotype level are limit...