[Enjoying research on lysozymes for 42 years].
نویسنده
چکیده
I have pursued research on lysozymes for 42 years. During that time, I made Several new findings, some of them by chance. My enjoyment of the following areas is reviewed: the story of tryptophan; protease digestion mechanisms; peptide mapping with RP-HPLC; gene engineering; renaturation of protein; catalytic residues; fluctuation and function; stabilization; folding; antigenecity; tolerance; and various lysozymes.
منابع مشابه
Quantitative immunological and electrophoretic comparison of primate lysozymes.
Rabbit antisera were produced against purified human and baboon lysozymes and tested by quantitative microcomplement hation for cross-reactivity with the lysozymes present in the milks of 19 primate species. No antigenic difference was found between the lysozymes of man and chimpanzee and almost none between those of man and orangutan. Major antigenic differences were detected between human lys...
متن کاملBi-functional activities of chimeric lysozymes constructed by domain swapping between bacteriophage T7 and K11 lysozymes.
The lysozymes encoded by bacteriophage T7 and K11 are both bifunctional enzymes sharing an extensive sequence homology (75%). The constructions of chimeric lysozymes were carried out by swapping the N-terminal and C-terminal domains between phage T7 and K11 lysozymes. This technique generated two chimeras, T7K11-lysozyme (N-terminal T7 domain and C-terminal K11 domain) and K11T7-lysozyme (N-ter...
متن کاملMidgut lysozymes of Lucilia sericata - new antimicrobials involved in maggot debridement therapy.
Larvae of Lucilia sericata are used for maggot debridement therapy (MDT) because of their ability to remove necrotic tissue and eradicate bacterial pathogens of infected wounds. So far, very few antibacterial factors have been fully characterized (eg lucifensin). Using a molecular approach, some other putative antimicrobial compounds, including three novel lysozymes, have been previously identi...
متن کاملCalcium-binding and structural stability of echidna and canine milk lysozymes.
For echidna and canine milk lysozymes, which were presumed to be the calcium-binding lysozymes by their amino acid sequences, we have quantitated their calcium-binding strength and examined their guanidine unfolding profiles. The calcium-binding constants of echidna and canine lysozymes were determined to be 8.6 x 10(6) M(-1) and 8.9 x 10(6) M(-1) in 0.1 M KCl at pH 7.1 and 20 C, respectively. ...
متن کاملPurification, characterization and comparison of reptile lysozymes.
Cation exchange column chromatography and gel filtration chromatography were used to purify four reptile lysozymes from egg white: SSTL A and SSTL B from soft shelled turtle (Trionyx sinensis), ASTL from Asiatic soft shelled turtle (Amyda cartilagenea) and GSTL from green sea turtle (Chelonia mydas). The molecular masses of the purified reptile lysozymes were estimated to be 14 kDa by SDS-PAGE....
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ورودعنوان ژورنال:
- Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
دوره 123 6 شماره
صفحات -
تاریخ انتشار 2003