Spin-Labeled Membranes
نویسنده
چکیده
In the next few minutes I shall present a very brief discussion of what the spin-label technique is, and how it may be used to study biological membranes, x, 2 A "spin label" is a synthetic organic paramagnetic free radical. Most spin
منابع مشابه
Spin-label ESR studies of lipid-protein interactions in thylakoid membranes.
Lipid-protein interactions in thylakoid membranes, and in the subthylakoid membrane fractions containing either photosystem 1 or photosystem 2, have been studied by using spin-labeled analogues of the thylakoid membrane lipid components, monogalactosyldiacylglycerol, phosphatidylglycerol, and phosphatidylcholine. The electron spin resonance spectra of the spin-labeled lipids all consist of two ...
متن کاملUniversity of Groningen Rotational mobility and orientational stability of a transport protein in lipid membranes
A single-cysteine mutant of the lactose transport protein LacS(C320A/W399C) from Streptococcus thermophilus was selectively labeled with a nitroxide spin label, and its mobility in lipid membranes was studied as a function of its concentration in the membrane by saturation-transfer electron spin resonance. Bovine rhodopsin was also selectively spin-labeled and studied to aid the interpretation ...
متن کاملRotational mobility and orientational stability of a transport protein in lipid membranes.
A single-cysteine mutant of the lactose transport protein LacS(C320A/W399C) from Streptococcus thermophilus was selectively labeled with a nitroxide spin label, and its mobility in lipid membranes was studied as a function of its concentration in the membrane by saturation-transfer electron spin resonance. Bovine rhodopsin was also selectively spin-labeled and studied to aid the interpretation ...
متن کاملRapid flip-flop of phospholipids in endoplasmic reticulum membranes studied by a stopped-flow approach.
The transbilayer movement of short-chain spin-labeled and fluorescent 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) phospholipid analogs in rat liver microsomes is measured by stopped-flow mixing of labeled microsomes with bovine serum albumin (BSA) solution. Extraction of analogs from the outer leaflet of microsomes to BSA can be directly monitored in conjunction with electron paramagnetic resonance...
متن کاملTransmembrane phospholipid motions induced by F glycoprotein in hemagglutinating virus of Japan.
Transfer of phospholipid from the envelope of hemagglutinating virus of Japan (HVJ) to erythrocyte (RBC) membrane and the virus-induced transfer of phospholipid between RBC membranes were studied using spin-labeled phosphatidylcholine (PC). The transfer of PC from membranes labeled densely with PC to unlabeled membranes was followed by the peak height increase in the electron spin resonance spe...
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عنوان ژورنال:
دوره 54 شماره
صفحات -
تاریخ انتشار 1969