Parallel single molecule detection with a fully integrated single-photon 2x2 CMOS detector array.

نویسندگان

  • Michael Gösch
  • Alexandre Serov
  • Tiemo Anhut
  • Theo Lasser
  • Alexis Rochas
  • Pierre-André Besse
  • Radivoje S Popovic
  • Hans Blom
  • Rudolf Rigler
چکیده

We present parallel single molecule detection (SMD) and fluorescence correlation spectroscopy (FCS) experiments with a fully integrated complementary metal oxide semiconductor (CMOS) single-photon 2x2 detector array. Multifocal excitation is achieved with a diffractive optical element (DOE). Special emphasis is placed on parallelization of the total system. The performance of the novel single-photon CMOS detector is investigated and compared to a state-of-the-art single-photon detecting module [having an actively quenched avalanche photodiode (APD)] by measurements on free diffusing molecules at different concentrations. Despite the order of magnitude lower detection efficiency of the CMOS detector compared to the state-of-the-art single-photon detecting module, we achieve single molecule sensitivity and reliably determine molecule concentrations. In addition, the CMOS detector performance for the determination of the fraction of slowly diffusing molecules in a primer solution (two-component analysis) is demonstrated. The potential of this new technique for high-throughput confocal-detection-based systems is discussed.

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عنوان ژورنال:
  • Journal of biomedical optics

دوره 9 5  شماره 

صفحات  -

تاریخ انتشار 2004