Characterization of two unusual guanylyl cyclases from dictyostelium.

Characterization of Two Unusual Guanylyl Cyclases from Dictyostelium*

Guanylyl cyclases across the tree of life.

This review explores the origins, diversity and functions of guanylyl cyclases in cellular organisms. In eukaryotes both cGMP and cAMP are produced by the conserved class III cyclase domains, while prokaryotes use five more unrelated catalysts for cyclic nucleotide synthesis. The class III domain is found embedded in proteins with a large variety of membrane topologies and other functional doma...

University of Groningen Regulation of Dictyostelium guanylyl cyclases

A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium.

Chemotactic stimulation of Dictyostelium cells results in a transient increase in cGMP levels, and transient phosphorylation of myosin II heavy and regulatory light chains. In Dictyostelium, two guanylyl cyclases and four candidate cGMP-binding proteins (GbpA- GbpD) are implicated in cGMP signalling. GbpA and GbpB are homologous proteins with a Zn2+-hydrolase domain. A double gbpA/gbpB gene dis...

Catalytic mechanism of the adenylyl and guanylyl cyclases: modeling and mutational analysis.

The adenylyl and guanylyl cyclases catalyze the formation of 3', 5'-cyclic adenosine or guanosine monophosphate from the corresponding nucleoside 5'-triphosphate. The guanylyl cyclases, the mammalian adenylyl cyclases, and their microbial homologues function as pairs of homologous catalytic domains. The crystal structure of the rat type II adenylyl cyclase C2 catalytic domain was used to model ...