Disassembly of a core-satellite nanoassembled substrate for colorimetric biomolecular detection.
نویسندگان
چکیده
The disassembly of a core-satellite nanostructured substrate is presented as a colorimetric biosensor observable under dark-field illumination. The fabrication method described herein utilizes thiol-mediated adsorption and streptavidin-biotin binding to self-assemble core-satellite nanostructures with a sacrificial linking peptide. Biosensing functionality is demonstrated with the protease trypsin, and the optical properties of the nanoassemblies are characterized. A figure of merit is presented to determine the optimal core and satellite size for visual detection. Nanoassemblies with 50 nm cores and 30 or 50 nm satellites are superior as these structures achieve an orange to green color shift greater than 70 nm that is easily discernible by the naked eye. This colorimetric substrate may prove to be a favorable alternative to liquid-based colloidal sensors and a useful visual readout mechanism for point-of-care microfluidic diagnostic assays.
منابع مشابه
The Dependency of Colorimetric Characteristics of Black Fabrics to the Whiteness Attribute of Substrate
In this paper the effect of whiteness attribute of white substrates on blackness property of black coated fabrics is investigated. To this aim, four cotton fabrics with different whiteness and tint attributes are used as white substrates. To prepare a set of black samples, various concentrations of four color pigments, i.e., red, green, blue and yellow, were mixed with the black one and applied...
متن کاملGeneric phosphatase activity detection using zinc mediated aggregation modulation of polypeptide-modified gold nanoparticles.
A challenge in the design of plasmonic nanoparticle-based colorimetric assays is that the change in colloidal stability, which generates the colorimetric response, is often directly linked to the biomolecular recognition event. New assay strategies are hence required for every type of substrate and enzyme of interest. Here, a generic strategy for monitoring of phosphatase activity is presented ...
متن کاملA visible light excitable colorimetric and fluorescent ESIPT probe for rapid and selective detection of hydrogen sulfide.
In this paper, a visible light excitable ESIPT probe was developed, which was found to be a very promising colorimetric and fluorescent sensor for rapid and selective detection of hydrogen sulfide in aqueous solution. The sensing mechanism and the application of this probe for hydrogen sulfide detection in biological serum and in simulated wastewater samples were investigated.
متن کاملColorimetric and fluorescent detection of protamines with an anionic polythiophene derivative.
We have developed a novel approach for the colorimetric and fluorescent detection of protamines based on an anionic polythiophene derivative. This probe has high sensitivity and selectivity toward protamines with a linear detectable range from 0.1 to 30 μg mL(-1), and can be used for protamine detection in serum samples.
متن کاملStimuli-responsive disassembly of nanoparticle aggregates for light-up colorimetric sensing.
Controlled assembly of nanomaterials has been the focus of much research. In contrast, controlled disassembly has not received much attention, even though both processes have been shown to be important in biology. By using a Pb2+-dependent RNA-cleaving DNAzyme, we demonstrate here control of the disassembly of gold nanoparticle aggregates in response to Pb2+. In the process, we show that nanopa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- ACS nano
دوره 5 7 شماره
صفحات -
تاریخ انتشار 2011