Factors that cause trimethoprim resistance in Streptococcus pyogenes.
نویسندگان
چکیده
The use of trimethoprim in treatment of Streptococcus pyogenes infections has long been discouraged because it has been widely believed that this pathogen is resistant to this antibiotic. To gain more insight into the extent and molecular basis of trimethoprim resistance in S. pyogenes, we tested isolates from India and Germany and sought the factors that conferred the resistance. Resistant isolates were identified in tests for trimethoprim or trimethoprim-sulfamethoxazole (SXT) susceptibility. Resistant isolates were screened for the known horizontally transferable trimethoprim-insensitive dihydrofolate reductase (dfr) genes dfrG, dfrF, dfrA, dfrD, and dfrK. The nucleotide sequence of the intrinsic dfr gene was determined for resistant isolates lacking the horizontally transferable genes. Based on tentative criteria, 69 out of 268 isolates (25.7%) from India were resistant to trimethoprim. Occurring in 42 of the 69 resistant isolates (60.9%), dfrF appeared more frequently than dfrG (23 isolates; 33.3%) in India. The dfrF gene was also present in a collection of SXT-resistant isolates from Germany, in which it was the only detected trimethoprim resistance factor. The dfrF gene caused resistance in 4 out of 5 trimethoprim-resistant isolates from the German collection. An amino acid substitution in the intrinsic dihydrofolate reductase known from trimethoprim-resistant Streptococcus pneumoniae conferred resistance to S. pyogenes isolates of emm type 102.2, which lacked other aforementioned dfr genes. Trimethoprim may be more useful in treatment of S. pyogenes infections than previously thought. However, the factors described herein may lead to the rapid development and spread of resistance of S. pyogenes to this antibiotic agent.
منابع مشابه
First Detection of Trimethoprim Resistance Determinant dfrG in Streptococcus pyogenes Clinical Isolates in India
Streptococcus pyogenes is capable of causing a wide spectrum of diseases, ranging from throat and skin infection to lifethreatening invasive diseases (2). The sequelae of streptococcal infections, such as acute rheumatic fever, represent a big health hazard (1). Recently, the emergence of invasive streptococcal diseases in India has been reported (5). Streptococcal emm types of the Indian subco...
متن کاملFirst Detection of Trimethoprim Resistance Determinant dfrG in Streptococcus pyogenes Clinical Isolates in India
Streptococcus pyogenes is capable of causing a wide spectrum of diseases, ranging from throat and skin infection to lifethreatening invasive diseases (2). The sequelae of streptococcal infections, such as acute rheumatic fever, represent a big health hazard (1). Recently, the emergence of invasive streptococcal diseases in India has been reported (5). Streptococcal emm types of the Indian subco...
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Objective(s): Streptococcus pyogenes, a notorious human pathogen is responsible to cause a wide range of infections varies from superficial common clinical illness to severe and life threatening infections. To our knowledge this is the first report exploring the emm types and superantigen/exotoxin gene profile of S. pyogenes from Pakistan. Materials a...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 58 4 شماره
صفحات -
تاریخ انتشار 2014