Animal and Veterinary Sciences

Seminal plasma transaminases (GOT and GPT) were assessed in diluted (Tris-egg yolk-citric acid-fructoseglycerol extender 1:4) and thawed (40 0 C for 15 seconds) semen of Sirohi bucks (control group). Diluted, cooled (from 25 0 C up to 5 0 C) and equilibrated (at 5 0 C for 2 hours) semen straws were frozen @ 15, 20, 25 and 30 0 C/minute for M1, M2, M3 and M4 groups respectively. These frozen straws were stored separately in LN2. Effect of freezing rates on leakage of tranferases was studied by assessing GOT and GPT in seminal plasma of thawed semen from M1, M2, M3 and M4 groups. The Mean + S.E. (C.V.%) values of transferases (GOT : GPT) in M1, M2, M3, M4 and control group were 313.00 + 8.40 (6.58) : 20.5 + 0.43 (5.12), 272.5 + 6.68 (6.01) : 16.33 + 1.02 (15.33), 255.00 + 10.1 (9.7) : 14.5 + 0.67 (11.33), 292.0 + 9.67 (8.09) : 19.67 + 0.70 (8.99) and 147.67 + 10.96 (18.17) ; 9.5 + 0.62 (15.96) units per 0.92 x 10 9 spermatozoa respectively. Least square analysis of variance revealed highly significant (P < 0.01) rise in the seminal plasma GOT enzyme levels in frozen thawed semen as compared to that in fresh diluted, cooled and equilibrated semen. Among the freezing mode groups lowest GOT and GPT values in seminal plasma was observed in M3 followed by M2, M4 and M1 groups in increasing order.