Theoretical and experimental demonstration of resolution beyond the Rayleigh limit by FINCH fluorescence microscopic imaging.
نویسندگان
چکیده
Fresnel Incoherent Correlation Holography (FINCH) enables holograms to be recorded from incoherent light with just a digital camera and spatial light modulator. We previously described its application to general three dimensional incoherent imaging and specifically to fluorescence microscopy, wherein one complex hologram contains the three dimensional information in the field of view, obviating the need for scanning or serial sectioning. We have now further analyzed FINCH in view of linear system theory and in comparison to conventional coherent and incoherent two dimensional imaging systems. We demonstrate, theoretically and experimentally, improved resolution by FINCH, when compared to conventional imaging.
منابع مشابه
Optimal resolution in Fresnel incoherent correlation holographic fluorescence microscopy
Fresnel Incoherent Correlation Holography (FINCH) enables holograms and 3D images to be created from incoherent light with just a camera and spatial light modulator (SLM). We previously described its application to microscopic incoherent fluorescence wherein one complex hologram contains all the 3D information in the microscope field, obviating the need for scanning or serial sectioning. We now...
متن کاملSuper-resolution imaging using the spatial-frequency filtered intensity fluctuation correlation
We report an experimental demonstration of a nonclassical imaging mechanism with super-resolving power beyond the Rayleigh limit. When the classical image is completely blurred out due to the use of a small imaging lens, by taking advantage of the intensity fluctuation correlation of thermal light, the demonstrated camera recovered the image of the resolution testing gauge. This method could be...
متن کاملEnhanced super resolution using Fresnel incoherent correlation holography with structured illumination.
The structured illumination (SI) technique has already been well established as a resolution enhancer in many studies and well demonstrated in many optical imaging systems during the past decade. The ability to use the SI in incoherent imaging systems was also introduced, especially in fluorescence microscopy. In this Letter, we propose and demonstrate a new approach to combine the SI technique...
متن کاملA statistical analysis of diffraction-limited imaging
The Rayleigh criterion is generally regarded as a fundamental limit and due to its practical accuracy in predicting the performance of optical imaging systems, it has unfortunately become accepted as a de-facto physical law. In this work, we will show that this limit is simply a very good rule of thumb, which under proper conditions typically related to the signal-to-noise (SNR) of the sensor, ...
متن کاملThree-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function.
We demonstrate single-molecule fluorescence imaging beyond the optical diffraction limit in 3 dimensions with a wide-field microscope that exhibits a double-helix point spread function (DH-PSF). The DH-PSF design features high and uniform Fisher information and has 2 dominant lobes in the image plane whose angular orientation rotates with the axial (z) position of the emitter. Single fluorescen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Optics express
دوره 19 27 شماره
صفحات -
تاریخ انتشار 2011