Amplification of the tetracycline resistance determinant of plasmid pAM alpha 1 in Streptococcus faecalis: dependence on host recombination machinery.
نویسندگان
چکیده
The plasmid pAM alpha 1 in Streptococcus faecalis was found to be severely impaired in its ability to exhibit amplification (generation of tandem repeats of the tetracycline resistance determinant during extended growth in the presence of tetracycline) when harbored by the recombination-deficient host cell UV202.
منابع مشابه
Physical and genetic analyses of streptococcal plasmid pAM beta 1 and cloning of its replication region.
Plasmid pAM beta 1, originally isolated from Streptococcus faecalis DS5, mediates resistance to the MLS (macrolide, lincosamide, and streptogramin B alpha) group of antibiotics. A restriction endonuclease map of the 26.5-kilobase (kb) pAM beta 1 molecule was constructed by using the enzymes AvaI, HpaII, EcoRI, PvuII, Kpn1, BstEII, HpaI, HhaI, and HindIII. A comparison of this map to those of fo...
متن کاملPlasmids, drug resistance, and gene transfer in the genus Streptococcus.
INTRODUCTION .......................................................... 409 PURIFUICATION OF PLASMID DEOXYRIBONUCLEIC ACID FROM STREPTCOCCI ......................................................... 412 CONJUGATION AND PLASMID TRANSFER ......... ........................ 413 SEX PHEROMONES IN STREPTOCOCCUS FAECALIS ....... ................ 415 RESISTANCE TRANSFER IN THE ABSENCE OF PLASMID DEOXYRI...
متن کاملPlasmid-determined tetracycline resistance in Streptococcus faecalis: tandemly repeated resistance determinants in amplified forms of pAMalpha1 DNA.
We recently reported that the six-megadalton plasmid pAMa (one of two plasmids in Stre#ococcusjaecaZis strain DS-5Cl) undergoes a gene amplification as a result of extended growth (many generations) of cells in the presence of a subinhibitory concentration of tetracycline. In this communication we present a detailed analysis of this phenomenon using the newly constructed S. faeealis strain DT-1...
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In this report, 30 tetracycline-resistant clinical isolates of group B Streptococcus were examined to assess the extent to which tetracycline resistance is plasmid mediated. Of these, 27 showed no physical or genetic evidence of plasmid-mediated resistance; however, one conjugative and two small (3.5 X 10(6)-dalton) multicopy non-self-transmissible tetracycline resistance plasmids were identifi...
متن کاملHelper plasmid cloning in Streptococcus sanguis: cloning of a tetracycline resistance determinant from the Streptococcus mutans chromosome.
A model system for testing the helper plasmid cloning system of Gryczan et al. (Mol. Gen. Genet. 177:459-467, 1980) was devised for the Streptococcus sanguis (Challis) host-vector system. In this system, linearized pVA736 plasmid efficiently transformed an S. sanguis (Challis) host containing a homologous plasmid, pVA380-1, but did not transform a plasmidless host or a host containing a nonhomo...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 143 2 شماره
صفحات -
تاریخ انتشار 1980